diff --git a/homeassistant/components/profiler/__init__.py b/homeassistant/components/profiler/__init__.py
index 9b4164c9f41..fab6932edd2 100644
--- a/homeassistant/components/profiler/__init__.py
+++ b/homeassistant/components/profiler/__init__.py
@@ -15,6 +15,7 @@ from homeassistant.components import persistent_notification
 from homeassistant.config_entries import ConfigEntry
 from homeassistant.const import CONF_SCAN_INTERVAL, CONF_TYPE
 from homeassistant.core import HomeAssistant, ServiceCall
+from homeassistant.exceptions import HomeAssistantError
 import homeassistant.helpers.config_validation as cv
 from homeassistant.helpers.event import async_track_time_interval
 from homeassistant.helpers.service import async_register_admin_service
@@ -269,6 +270,11 @@ async def _async_generate_memory_profile(hass: HomeAssistant, call: ServiceCall)
     # Imports deferred to avoid loading modules
     # in memory since usually only one part of this
     # integration is used at a time
+    if sys.version_info >= (3, 11):
+        raise HomeAssistantError(
+            "Memory profiling is not supported on Python 3.11. Please use Python 3.10."
+        )
+
     from guppy import hpy  # pylint: disable=import-outside-toplevel
 
     start_time = int(time.time() * 1000000)
diff --git a/homeassistant/components/profiler/manifest.json b/homeassistant/components/profiler/manifest.json
index 11896c02fdd..81eb77537fb 100644
--- a/homeassistant/components/profiler/manifest.json
+++ b/homeassistant/components/profiler/manifest.json
@@ -5,5 +5,9 @@
   "config_flow": true,
   "documentation": "https://www.home-assistant.io/integrations/profiler",
   "quality_scale": "internal",
-  "requirements": ["pyprof2calltree==1.4.5", "guppy3==3.1.2", "objgraph==3.5.0"]
+  "requirements": [
+    "pyprof2calltree==1.4.5",
+    "guppy3==3.1.2;python_version<'3.11'",
+    "objgraph==3.5.0"
+  ]
 }
diff --git a/requirements_all.txt b/requirements_all.txt
index 13b0e6874f0..9cc38659d9c 100644
--- a/requirements_all.txt
+++ b/requirements_all.txt
@@ -849,7 +849,7 @@ gspread==5.5.0
 gstreamer-player==1.1.2
 
 # homeassistant.components.profiler
-guppy3==3.1.2
+guppy3==3.1.2;python_version<'3.11'
 
 # homeassistant.components.iaqualink
 h2==4.1.0
diff --git a/requirements_test_all.txt b/requirements_test_all.txt
index 78e49f2b24e..03b0a9ec0fc 100644
--- a/requirements_test_all.txt
+++ b/requirements_test_all.txt
@@ -644,7 +644,7 @@ growattServer==1.3.0
 gspread==5.5.0
 
 # homeassistant.components.profiler
-guppy3==3.1.2
+guppy3==3.1.2;python_version<'3.11'
 
 # homeassistant.components.iaqualink
 h2==4.1.0
diff --git a/script/hassfest/requirements.py b/script/hassfest/requirements.py
index 8e4ac524b2f..8b9f73336fe 100644
--- a/script/hassfest/requirements.py
+++ b/script/hassfest/requirements.py
@@ -90,7 +90,7 @@ def validate_requirements_format(integration: Integration) -> bool:
         if not version:
             continue
 
-        for part in version.split(","):
+        for part in version.split(";", 1)[0].split(","):
             version_part = PIP_VERSION_RANGE_SEPARATOR.match(part)
             if (
                 version_part
diff --git a/tests/components/profiler/test_init.py b/tests/components/profiler/test_init.py
index a83bd3b2b1b..636067341e1 100644
--- a/tests/components/profiler/test_init.py
+++ b/tests/components/profiler/test_init.py
@@ -1,6 +1,7 @@
 """Test the Profiler config flow."""
 from datetime import timedelta
 import os
+import sys
 from unittest.mock import patch
 
 import pytest
@@ -18,6 +19,7 @@ from homeassistant.components.profiler import (
 from homeassistant.components.profiler.const import DOMAIN
 from homeassistant.const import CONF_SCAN_INTERVAL, CONF_TYPE
 from homeassistant.core import HomeAssistant
+from homeassistant.exceptions import HomeAssistantError
 import homeassistant.util.dt as dt_util
 
 from tests.common import MockConfigEntry, async_fire_time_changed
@@ -53,6 +55,9 @@ async def test_basic_usage(hass: HomeAssistant, tmpdir) -> None:
     await hass.async_block_till_done()
 
 
+@pytest.mark.skipif(
+    sys.version_info >= (3, 11), reason="not yet available on python 3.11"
+)
 async def test_memory_usage(hass: HomeAssistant, tmpdir) -> None:
     """Test we can setup and the service is registered."""
     test_dir = tmpdir.mkdir("profiles")
@@ -83,6 +88,24 @@ async def test_memory_usage(hass: HomeAssistant, tmpdir) -> None:
     await hass.async_block_till_done()
 
 
+@pytest.mark.skipif(sys.version_info < (3, 11), reason="still works on python 3.10")
+async def test_memory_usage_py311(hass: HomeAssistant, tmpdir) -> None:
+    """Test raise an error on python3.11."""
+    entry = MockConfigEntry(domain=DOMAIN)
+    entry.add_to_hass(hass)
+
+    assert await hass.config_entries.async_setup(entry.entry_id)
+    await hass.async_block_till_done()
+    assert hass.services.has_service(DOMAIN, SERVICE_MEMORY)
+    with pytest.raises(
+        HomeAssistantError,
+        match="Memory profiling is not supported on Python 3.11. Please use Python 3.10.",
+    ):
+        await hass.services.async_call(
+            DOMAIN, SERVICE_MEMORY, {CONF_SECONDS: 0.000001}, blocking=True
+        )
+
+
 async def test_object_growth_logging(
     hass: HomeAssistant, caplog: pytest.LogCaptureFixture
 ) -> None:
diff --git a/tests/hassfest/test_requirements.py b/tests/hassfest/test_requirements.py
index cb73e5b9603..174bf841788 100644
--- a/tests/hassfest/test_requirements.py
+++ b/tests/hassfest/test_requirements.py
@@ -52,6 +52,7 @@ def test_validate_requirements_format_ignore_pin_for_custom(integration: Integra
         "test_package~=0.5.0",
         "test_package>=1.4.2,<1.4.99,>=1.7,<1.8.99",
         "test_package>=1.4.2,<1.9,!=1.5",
+        "test_package>=1.4.2;python_version<'3.11'",
     ]
     integration.path = Path("")
     assert validate_requirements_format(integration)