diff --git a/homeassistant/components/file/__init__.py b/homeassistant/components/file/__init__.py
index 9e91aa07103..aa3e241cc81 100644
--- a/homeassistant/components/file/__init__.py
+++ b/homeassistant/components/file/__init__.py
@@ -2,7 +2,13 @@
 
 from homeassistant.components.notify import migrate_notify_issue
 from homeassistant.config_entries import SOURCE_IMPORT, ConfigEntry
-from homeassistant.const import CONF_FILE_PATH, CONF_NAME, CONF_PLATFORM, Platform
+from homeassistant.const import (
+    CONF_FILE_PATH,
+    CONF_NAME,
+    CONF_PLATFORM,
+    CONF_SCAN_INTERVAL,
+    Platform,
+)
 from homeassistant.core import DOMAIN as HOMEASSISTANT_DOMAIN, HomeAssistant
 from homeassistant.exceptions import ConfigEntryNotReady
 from homeassistant.helpers import (
@@ -63,6 +69,8 @@ async def async_setup(hass: HomeAssistant, config: ConfigType) -> bool:
             if item[CONF_PLATFORM] == DOMAIN:
                 file_config_item = IMPORT_SCHEMA[domain](item)
                 file_config_item[CONF_PLATFORM] = domain
+                if CONF_SCAN_INTERVAL in file_config_item:
+                    del file_config_item[CONF_SCAN_INTERVAL]
                 hass.async_create_task(
                     hass.config_entries.flow.async_init(
                         DOMAIN,
diff --git a/tests/components/file/test_sensor.py b/tests/components/file/test_sensor.py
index d2059f4d564..60a81df2b1e 100644
--- a/tests/components/file/test_sensor.py
+++ b/tests/components/file/test_sensor.py
@@ -21,6 +21,7 @@ async def test_file_value_yaml_setup(
     config = {
         "sensor": {
             "platform": "file",
+            "scan_interval": 30,
             "name": "file1",
             "file_path": get_fixture_path("file_value.txt", "file"),
         }